Two men have been taken into custody, one accused of sexually assaulting a female passenger on a bus in Waurn Ponds.
Witnesses reported the incident on the bus travelling towards Waurn Ponds Railway Station around 11.50am on Wednesday.
Officers used pepper spray to arrest the 41-year-old Colac man when the bus stopped on Sugrargum Drive.
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A 37-year-old Colac man was arrested for resisting police.
Both men were taken into custody to be interviewed.
